| Klein Brujo was found to be a promising Argentine spring wheat genotype for transformation studies. In the present work we optimized the biolistic transformation of embryogenic scutellar calli from this genotype. We first identified scutellar callus induction media (SCIM) most promising for in vitro embryogenic plant regeneration with Klein Brujo. We then co-bombarded embryos of Klein Brujo and, for comparison, of Bobwhite, a highly transformable wheat line, on these media with 1 μm gold particles coated with two plasmids. One of these contained the marker gene gfp (linked to the CaMV35S-promoter) and the selection gene bar (resistance to phosphinothricin: PPT, linked to the maize Ubi1-promoter), whereas the other contained ipt (encoding... |
